📇 Rhumatologue ·

M FRANCOIS ROUSSET

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4 raisons identifiées

Auteur de référence en rhumatologie
50 articles scientifiques publiés — un praticien à la pointe de la recherche
Encadrant universitaire
Forme la prochaine génération de rhumatologues (6 thèses dirigées)
Référence presse grand public
Cité 20 fois dans les médias — pédagogie reconnue
Expérience confirmée
33 ans d'exercice en rhumatologie — recul clinique solide

Rhumatologue

RPPS 10001773943

EnseignementExpérience confirmée

33ans d'exercice (thèse 1993)

📊 Reconnaissance scientifique : 76/100📝 327 articles publiés🇫🇷 Publications académiques françaises (8)🎓 6 thèses dirigées

✨ Génération du profil synthétique IA en cours…

Données ANS publiques (Licence Ouverte 2.0) · Enrichissements MonRhumato 100 % opt-in · Toute personne référencée peut demander la suppression ou la rectification.

Top publications · les plus citées

Phages and their satellites encode hotspots of antiviral systems

Cell host & microbe · 2022

📚 248 citations🎯 RCR 20.63Top 1% NIH🔓 Open Access📄 PDF gratuit ↗

Adaptive responses of animals to climate change are most likely insufficient

Nature communications · 2019

📚 230 citations🎯 RCR 13.92Top 1% NIH🔓 Open Access📄 PDF gratuit ↗

Lire l'abstract Crossref ↓

Abstract Biological responses to climate change have been widely documented across taxa and regions, but it remains unclear whether species are maintaining a good match between phenotype and environment, i.e. whether observed trait changes are adaptive. Here we reviewed 10,090 abstracts and extracted data from 71 studies reported in 58 relevant publications, to assess quantitatively whether phenotypic trait changes associated with climate change are adaptive in animals. A meta-analysis focussing on birds, the taxon best represented in our dataset, suggests that global warming has not systematically affected morphological traits, but has advanced phenological traits. We demonstrate that these advances are adaptive for some species, but imperfect as evidenced by the observed consistent selection for earlier timing. Application of a theoretical model indicates that the evolutionary load imposed by incomplete adaptive responses to ongoing climate change may already be threatening the persistence of species.

A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9

Nature communications · 2018

📚 215 citations🎯 RCR 8.01Top 4% NIH🔓 Open Access📄 PDF gratuit ↗

Lire l'abstract Crossref ↓

AbstractHigh-throughput CRISPR-Cas9 screens have recently emerged as powerful tools to decipher gene functions and genetic interactions. Here we use a genome-wide library of guide RNAs to direct the catalytically dead Cas9 (dCas9) to block gene transcription in Escherichia coli. Using a machine-learning approach, we reveal that guide RNAs sharing specific 5-nucleotide seed sequences can produce strong fitness defects or even kill E. coli regardless of the other 15 nucleotides of guide sequence. This effect occurs at high dCas9 concentrations and can be alleviated by tuning the expression of dCas9 while maintaining strong on-target repression. Our results also highlight the fact that off-targets with as little as nine nucleotides of homology to the guide RNA can strongly block gene expression. Altogether this study provides important design rules to safely use dCas9 in E. coli.

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M FRANCOIS ROUSSET

Pourquoi vous devez consulter ce praticien

Thèses universitaires

Direction de thèses

Activité de recherche & publications

Publications académiques en France

Lieu de consultation

Tarifs & secteur de conventionnement

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Articles de presse (20)

Top publications · les plus citées

Publications scientifiques (50) — classées par pathologie

Transversal44

Génétique5

Fièvres auto-inflammatoires1

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