📇 Rhumatologue ·
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Auteur de référence en rhumatologie
36 articles scientifiques publiés — un praticien à la pointe de la recherche
✨ Génération du profil synthétique IA en cours…
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Hepatology (Baltimore, Md.) · 1998
The detection of antimitochondrial antibodies (AMAs) is an important criterion for the diagnosis of primary biliary cirrhosis (PBC). During the last decade, the mitochondrial autoantigens have been cloned, sequenced, and identified as members of the 2-oxo-acid dehydrogenase pathway, including the E2 subunits of pyruvate dehydrogenase (PDC-E2), branched-chain 2-oxo-acid dehydrogenase (BCOADC-E2), and 2-oxo-glutarate dehydrogenase (OGDC-E2). We have developed a rapid and sensitive diagnostic test for use in PBC based on a triple hybrid recombinant molecule (r-MIT3) that contains the autoepitopes of PDC-E2, BCOADC-E2, and OGDC-E2. To help understand the frequency and antigen specificity of AMAs in an asymptomatic population and to identify patients with early disease, we investigated the prevalence of AMA, by enzyme-linked immunosorbent assay (ELISA), in a cohort of 1,530 people from northern Italy. Positive sera were further analyzed for immunoglobulin (Ig) isotypes, subclasses, and epitopes of AMA by a combination of ELISA and immunoblotting. In this cohort of 1,530 people, 9 (0.5%) reacted to r-MIT3 by ELISA. Of the 9 reactive sera, 2 recognized PDC-E2, 2 of 9 recognized BCOADC-E2, 1 of 9 recognized OGDC-E2, 2 of 9 recognized both PDC-E2 and BCOADC-E2, and 1 of 9 recognized PDC-E2 and OGDC-E2. AMA reactivity was primarily IgM and IgA. Epitope mapping revealed an AMA pattern of reactivity to PDC-E2 that differed from that found in patients with histologically proven PBC in most of the sera. However, 1 sera of a 72-year-old female with a normal alkaline phosphatase had an AMA profile identical to typical PBC. After a variable follow-up period (8-14 months), sera from 8 of 9 of these people were re-obtained for AMA and relative epitope mapping. Interestingly, the reactivity had a wider AMA pattern than before.
Journal of clinical and diagnostic research : JCDR · 2014
Haematologica · 2007
Source PubMed · Recherche par auteur (homonymes possibles, vérifier l'affiliation).
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Caviglia GP, Abate ML, Manzini P, Danielle F, et al.
Haematologica · 2007 · Journal Article
Manzini P, Girotto M, Borsotti R, Giachino O, et al.
Biochemical and biophysical research communications · 2006 · Journal Article
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Enfermedades infecciosas y microbiologia clinica · 2000 · Case Reports
Fumagalli J, Danielle A, Shinzato R, Trombetta L, et al.
Journal of clinical microbiology · 1998 · Journal Article
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Hepatology (Baltimore, Md.) · 1998 · Journal Article
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International journal of epidemiology · 1994 · Journal Article
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Oxford medical case reports · 2025 · Case Reports
Gupta A, D V, Nawaz A, Hari V
AAPS PharmSciTech · 2019 · Journal Article
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Journal of psychosocial nursing and mental health services · 1999 · Journal Article
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Sleep · 2018 · Journal Article
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