📇 Rhumatologue · PARIS · (75) · Salarié

M Bertrand DUCLOS

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5 raisons identifiées

Auteur de référence en rhumatologie
22 articles scientifiques publiés — un praticien à la pointe de la recherche
Encadrant universitaire
Forme la prochaine génération de rhumatologues (1 thèse dirigée)
En plein centre-ville
PARIS (75002) — accessible depuis tout le bassin urbain
Référence presse grand public
Cité 2 fois dans les médias — pédagogie reconnue
Délais de RDV courts dans la région
336.2 rhumatos / 100 000 hab. — département bien doté

Rhumatologue

📍 PARIS (75)SalariéRPPS 10001121440

🇫🇷 Publications académiques françaises (7)🎓 1 thèse dirigée

✨ Génération du profil synthétique IA en cours…

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🎓 1 thèse dirigée

Caractérisation de nouveaux substrats de la sérine/thréonine kinase Stk1 de Staphylococcus aureus
2012
Marie-Ève Cluzel

Données ANS publiques (Licence Ouverte 2.0) · Enrichissements MonRhumato 100 % opt-in · Toute personne référencée peut demander la suppression ou la rectification.

Top publications · les plus citées

Identification of N-{cis-3-[Methyl(7H-pyrrolo[2,3-d]pyrimidin-4-yl)amino]cyclobutyl}propane-1-sulfonamide (PF-04965842): A Selective JAK1 Clinical Candidate for the Treatment of Autoimmune Diseases

Journal of medicinal chemistry · 2018

📚 129 citations🎯 RCR 5.29Top 7% NIH🔓 Open Access📄 PDF gratuit ↗

Characterization of a serine/threonine kinase involved in virulence of Staphylococcus aureus

Journal of bacteriology · 2009

📚 110 citations🎯 RCR 2.89Top 17% NIH🔓 Open Access

Lire l'abstract Crossref ↓

ABSTRACT Staphylococcus aureus is a common human cutaneous and nasal commensal and a major life-threatening pathogen. Adaptation to the different environments encountered inside and outside the host is a crucial requirement for survival and colonization. We identified and characterized a eukaryotic-like serine/threonine kinase with three predicted extracellular PASTA domains (SA1063, or Stk1) and its associated phosphatase (SA1062, or Stp1) in S. aureus . Biochemical analyses revealed that Stk1 displays autokinase activity on threonine and serine residues and is localized to the membrane. Stp1 is a cytoplasmic protein with manganese-dependent phosphatase activity toward phosphorylated Stk1. In-frame deletions of the stk1 and stp1 genes were constructed in S. aureus strain 8325-4. Phenotypic analyses of the mutants revealed reduced growth of the stk1 mutant in RPMI 1640 defined medium that was restored when adenine was added to the medium. Furthermore, the stk1 mutant displayed increased resistance to Triton X-100 and to fosfomycin, suggesting modifications in cell wall metabolism. The stk1 mutant was tested for virulence in a mouse pyelonephritis model and found to be strongly reduced for survival in the kidneys (approximately 2-log-unit decrease) compared to the parental strain. Renal histopathological analyses showed severe inflammatory lesions in mice infected with the parental S. aureus SH1000 strain, whereas the Δ stk1 mutant led to only minimal renal lesions. These results confirm the important role of Stk1 for full expression of S. aureus pathogenesis and suggest that phosphorylation levels controlled by stk1 are essential in controlling bacterial survival within the host.

Low prevalence of antibodies to glucose-6-phosphate isomerase in patients with rheumatoid arthritis and a spectrum of other chronic autoimmune disorders

Arthritis and rheumatism · 2003

📚 103 citations🎯 RCR 2.20Top 24% NIH

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AbstractObjectiveArthritis in the K/BxN mouse model results from pathogenic immunoglobulins that recognize glucose‐6‐phosphate isomerase (GPI), a glycolytic enzyme residing in the cytoplasm of all cells. Antibodies directed against GPI can, alone, transfer arthritis to healthy recipients. Previous experiments have revealed significant titers of anti‐GPI antibodies in the serum of many patients with rheumatoid arthritis (RA). We evaluated the generality of these observations in cohorts of patients with 12 different arthritic and chronic autoimmune diseases and in population‐matched healthy control subjects.MethodsAnti‐GPI antibodies were assayed in 811 individual serum samples by enzyme‐linked immunosorbent assay with 2 forms of GPI, recombinant and native. Results were confirmed by immunoblotting.ResultsSeveral patients had significantly elevated anti‐GPI antibody titers, but without the prevalence or the specificity reported previously. Only 15% of RA patients had anti‐GPI antibodies (range 12–29% in different cohorts), with a higher prevalence in patients with active disease. Psoriatic arthritis, undifferentiated arthritis, and spondylarthropathy patients also displayed anti‐GPI antibodies at similar frequencies (12–25%). Similar titers were detected in a proportion (5–10%) of control subjects or patients with Crohn's disease or sarcoidosis. Very high titers were found in rare cases of RA and systemic lupus erythematosus.ConclusionNo disease‐specific pattern of antibody positivity to GPI was apparent. While the antibody‐mediated mechanism at play in the mouse model may exemplify a generic mechanism for some forms of arthritis in humans, GPI itself does not appear to be a target common to the majority of RA patients.

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M Bertrand DUCLOS

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Direction de thèses

Publications académiques en France

Lieu de consultation

Tarifs & secteur de conventionnement

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Articles de presse (2)

Top publications · les plus citées

Publications scientifiques (22) — classées par pathologie

Transversal19

Épidémiologie & registres2

Vascularites1

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