📇 Rhumatologue · PARIS CEDEX 15 · (75) · Salarié

M DOMINIQUE PRIE

Pourquoi vous devez consulter ce praticien

4 raisons identifiées

Auteur de référence en rhumatologie
20 articles scientifiques publiés — un praticien à la pointe de la recherche
Encadrant universitaire
Forme la prochaine génération de rhumatologues (2 thèses dirigées)
Expérience confirmée
32 ans d'exercice en rhumatologie — recul clinique solide
Délais de RDV courts dans la région
336.2 rhumatos / 100 000 hab. — département bien doté

Rhumatologue

📍 PARIS CEDEX 15 (75)SalariéRPPS 10000527746

EnseignementExpérience confirmée

32ans d'exercice (thèse 1994)

📊 Reconnaissance scientifique : 44/100📝 505 articles publiés🇫🇷 Publications académiques françaises (8)🎓 2 thèses dirigées

Données ANS publiques (Licence Ouverte 2.0) · Enrichissements MonRhumato 100 % opt-in · Toute personne référencée peut demander la suppression ou la rectification.

Top publications · les plus citées

High-dose biotin therapy leading to false biochemical endocrine profiles: validation of a simple method to overcome biotin interference

Clinical chemistry and laboratory medicine · 2017

📚 97 citations🎯 RCR 5.73Top 6% NIH

Lire l'abstract Crossref ↓

Abstract Background: High-dose biotin therapy is beneficial in progressive multiple sclerosis (MS) and is expected to be adopted by a large number of patients. Biotin therapy leads to analytical interference in many immunoassays that utilize streptavidin-biotin capture techniques, yielding skewed results that can mimic various endocrine disorders. We aimed at exploring this interference, to be able to remove biotin and avoid misleading results. Methods: We measured free triiodothyronine (fT3), free thyroxine (fT4), thyroid-stimulating hormone (TSH), parathyroid homrone (PTH), 25-hydroxyvitamin D (25OHD), follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin, C-peptide, cortisol (Roche Diagnostics assays), biotin and its main metabolites (liquid chromatography tandem mass spectrometry) in 23 plasmas from MS patients and healthy volunteers receiving high-dose biotin, and in 39 biotin-unsupplemented patients, before and after a simple procedure (designated N5) designed to remove biotin by means of streptavidin-coated microparticles. We also assayed fT4, TSH and PTH in the 23 high-biotin plasmas using assays not employing streptavidin-biotin binding. Results: The biotin concentration ranged from 31.7 to 1160 µg/L in the 23 high-biotin plasmas samples. After the N5 protocol, the biotin concentration was below the detection limit in all but two samples (8.3 and 27.6 μg/L). Most hormones results were abnormal, but normalized after N5. All results with the alternative methods were normal except two slight PTH elevations. In the 39 biotin-unsupplemented patients, the N5 protocol did not affect the results for any of the hormones, apart from an 8.4% decrease in PTH. Conclusions: We confirm that most streptavidin-biotin hormone immunoassays are affected by high biotin concentrations, leading to a risk of misdiagnosis. Our simple neutralization method efficiently suppresses biotin interference.

Hormone Immunoassay Interference: A 2021 Update

Annals of laboratory medicine · 2022

📚 83 citations🎯 RCR 11.21Top 2% NIH🔓 Open Access

Physiology of FGF23 and overview of genetic diseases associated with renal phosphate wasting

Metabolism: clinical and experimental · 2020

📚 60 citations🎯 RCR 4.11Top 10% NIH🔓 Open Access📄 PDF gratuit ↗

Cookies & analyse de trafic

M DOMINIQUE PRIE

Pourquoi vous devez consulter ce praticien

Thèses universitaires

Direction de thèses

Activité de recherche & publications

Publications académiques en France

Lieu de consultation

Tarifs & secteur de conventionnement

Prendre rendez-vous & contact

Top publications · les plus citées

Publications scientifiques (20) — classées par pathologie

Transversal10

Essai clinique5

Pédiatrie2

Épidémiologie & registres1

Génétique1

IRM ostéo-articulaire1

Pharmacovigilance1

Risque cardio-vasculaire1

Partager cette fiche