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Délais de RDV courts dans la région
134 rhumatos / 100 000 hab. — département bien doté
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CABINET DU DR ERIC FABIEN
18 RUE DU COMMANDANT L HERMINIER, 94240 L HAY LES ROSES
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Lien Doctolib = recherche Google site:doctolib.fr (le 1er résultat est presque toujours le profil correct s'il existe).
Journal of parasitology research · 2012
Objective. To determine the prevalence and the genotypes ofEnterocytozoon bieneusiin stool specimens from HIV patients.Methods. This cross-sectional study was carried out in Kinshasa hospitals between 2009 and 2012. Detection of microsporidia includingE. bieneusiandE. intestinaliswas performed in 242 HIV-infected patients. Typing was based on DNA polymorphism of the ribosomal DNA ITS region ofE. bieneusi. PCRRFLP generated with two restriction enzymes (Nla III and Fnu 4HI) in PCR-amplified ITS products for classifying strains into different lineages. The diagnosis performance of the indirect immune-fluorescence-monoclonal antibody (IFI-AcM) was defined in comparison with real-time PCR as the gold standard.Results. Out of 242 HIV-infected patients, using the real-time PCR, the prevalence ofE. bieneusiwas 7.9% (n=19) among the 19E. bieneusi, one was coinfected withE. intestinalis. In 19E. bieneusipersons using PCR-RFLP method, 5 type I strains ofE. bieneusi(26.3%) and 5 type IV strains ofE. bieneusi(26.3%) were identified. The sensitivity of IFI-AcM was poor as estimated 42.1%.Conclusion. Despite different PCR methods, there is possible association between HIVinfection, geographic location (France, Cameroun, Democratic Republic of Congo), and the concurrence of type I and type IV strains.
Environmental microbiology reports · 2025
ABSTRACT During an 18‐month longitudinal study, bi‐monthly water samples were taken upstream and downstream of a watershed. In order to detect carbapenem‐resistant E. coli , the CHROMIDCarba medium was used. Of the 863 isolates collected from 144 samples, E. coli identification was confirmed for only seven of them, isolated on the same day. For six isolates, a slightly reduced susceptibility to carbapenems was observed. The results of the whole genome sequencing indicate that the six isolates belong to the same clone (O8:H7, ST196). Furthermore, a mutation of the ompC porin coupled with the presence of the bla CMY‐2 gene, on an IncI1 plasmid, would be at the origin of the reduced sensitivity of these strains to carbapenems. This type of mechanism has already been described in human clinical cases. To our knowledge, this is the first time that it has been identified in strains from the aquatic environment. The detection of E. coli with reduced susceptibility to carbapenems one time in 18 months (one out of 36 sampling dates) could be considered a one‐time event. However, this illustrates the importance of monitoring the aquatic environment but also the methodological difficulties of such surveillance due to the poor efficacy of the isolation method.
Source PubMed · Recherche par auteur (homonymes possibles, vérifier l'affiliation).
Journal of parasitology research · 2012 · Journal Article
Wumba R, Jean M, Benjamin LM, Madone M, et al.
Environmental microbiology reports · 2025 · Journal Article
Laëtitia LD, Mai-Lan T, Eric J, Fabien V, et al.